Til hovedinnhold
English
Publikasjoner

Diversity and dynamics of marine haptophytes as revealed by 454 pyrosequencing

Vitenskapelig foredrag
Publiseringsår
2012
Eksterne nettsted
Cristin
Prosjekt
Forfattere
Bente Edvardsen, Elianne Sirnæs Egge, Lucie Bittner, Wenche Eikrem, Stéphane Audic, Tom Andersen, Sebastien Santini, Hervé Moreau, Colomban de Vargas

Sammendrag

Haptophytes are microalgae and a major component of marine plankton communities. Our aim is to provide new knowledge on the diversity and distribution of haptophytes below the genus-level, and how this is affected by environmental factors. We have developed and tested methods for analysing diversity using 454-pyrosequencing, We designed haptophyte-specific rDNA primers and constructed haptophyte databases and phylogenies for taxonomic assignation. We sequenced an artificial haptophyte community and compared the diversity and relative abundances from DNA and RNA/cDNA using various rDNA primers, and different programs to 18 remove errors from sequence data. We applied 454-pyrosequencing to samples from six European coastal sites and seasonal samples from Oslofjorden, and combined this with electron microscopy. We propose a strategy to more accurately depict the haptophyte diversity using 454-pyrosequencing and present results using this strategy.