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Diatom diversity through HTS-metabarcoding in coastal European seas

Vitenskapelig artikkel
Publiseringsår
2018
Tidsskrift
Scientific Reports
Eksterne nettsted
Cristin
Fulltekst
Prosjekt
Arkiv
Doi
NIVA-involverte
Wenche Eikrem
Forfattere
Roberta Piredda, Jean-Michel Claverie, Johan Decelle, Colomban de Vargas, Micah Dunthorn, Bente Edvardsen, Wenche Eikrem, D. Forster, Wiebe Koistra, Ramiro Logares, Ramón Massana, Marina Montresor, Fabrice Not, Hiroyuki Ogata, Jan M. Pawlowski, Sarah Romac, Diana Sarno, Thorsten Stoeck, Adriana Zingone

Sammendrag

Diatoms constitute a diverse lineage of unicellular organisms abundant and ecologically important in aquatic ecosystems. Compared to other protists, their biology and taxonomy are well-studied, offering the opportunity to combine traditional approaches and new technologies. We examined a dataset of diatom 18S rRNA- and rDNA- (V4 region) reads from different plankton size-fractions and sediments from six European coastal marine sites, with the aim of identifying peculiarities and commonalities with respect to the whole protistan community. Almost all metabarcodes (99.6%) were assigned to known genera (121) and species (236), the most abundant of which were those already known from classic studies and coincided with those seen in light microscopy. rDNA and rRNA showed comparable patterns for the dominant taxa, but rRNA revealed a much higher diversity particularly in the sediment communities. Peculiar to diatoms is a tight bentho-pelagic coupling, with many benthic or planktonic species colonizing both water column and sediments and the dominance of planktonic species in both habitats. Overall metabarcoding results reflected the marked specificity of diatoms compared to other protistan groups in terms of morphological and ecological characteristics, at the same time confirming their great potential in the description of protist communities.